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. 2015 Aug 13;290(41):25012–25022. doi: 10.1074/jbc.M115.674796

FIGURE 2.

FIGURE 2.

SPBB1 co-localizes with TbPLK in the basal body during early cell cycle stages. A, subcellular localization of 3HA-SPBB1 during the cell cycle. SPBB1 was endogenously tagged with an N-terminal triple HA epitope. Cells were co-immunostained with the FITC-conjugated anti-HA mAb and anti-TbSAS-6 pAb, and then counterstained with DAPI for DNA. DIC, differential interference contrast. Scale bar: 5 μm. B, localization of 3HA-SPBB1 and TbPLK during the cell cycle. Cells were co-immunostained with the FITC-conjugated anti-HA mAb and anti-TbPLK pAb, and then counterstained with DAPI for DNA. Scale bar: 5 μm. C, co-localization of PTP-SPBB1 and TbPLK-3HA in the basal body. SPBB1 was endogenously tagged with the PTP epitope at the N terminus in the same cell line expressing endogenously 3HA-tagged TbPLK. Cells were co-immunostained with the FITC-conjugated anti-HA mAb and anti-protein A pAb to detect TbPLK-3HA and PTP-SPBB1, respectively. Scale bar: 5 μm.