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. 2015 Aug 13;290(41):25012–25022. doi: 10.1074/jbc.M115.674796

FIGURE 3.

FIGURE 3.

SPBB1 RNAi causes cytokinesis defects and flagellum detachment. A, RNAi of SPBB1 caused growth defects. Inset shows the Western blot to monitor the level of PTP-SPBB1, which was endogenously tagged in the SPBB1 RNAi cell line, before and after tetracycline (Tet) induction of RNAi. The level of the α-6 subunit of the 26S proteasome (TbPSA6) was included as the loading control. B, tabulation of cells with different numbers of kinetoplast (K) and nucleus (N) before and after SPBB1 RNAi for up to 4 days. About 200 cells were counted from each time point. Error bars represent S.D. calculated from three independent experiments. C, the percentage of cells with detached flagellum upon SPBB1 RNAi. About 200 cells were counted from each time point, and error bars represent S.D. calculated from three independent experiments. ***, p < 0.001. D, morphology of SPBB1 RNAi cells, showing the 2N1K and XN1K cells with a detached new flagellum (arrows). DIC, differential interference contrast. Scale bar: 5 μm.