FIGURE 6.

Stopped-flow kinetics of the SQR reaction with sulfite. A, reaction between 10 μm SQR and 200 μm sulfite in 50 mm potassium phosphate buffer, pH 7.4, with 0.03% DHPC, monitored over a period of 75 s. B, kinetics of CT formation in the absence and presence of 30 μm CoQ₁. The kinetic traces could be fitted to a two-exponential model as described under “Results.” The addition of CoQ₁ did not significantly alter the kinetics of CT complex formation, and similar kinetics were observed in 50 mm Tris-Cl buffer, pH 8.0.