FIGURE 6.

Mutation of specific α subunit knuckle domain residues diminished the Na⁺ self-inhibition response. Representative current traces for Na⁺ self-inhibition responses were from oocytes expressing either WT αβγ or mutant α subunits with WT β and γ subunits. The mutated residues are shown above each panel. Oocytes were clamped at −60 or −100 mV, and whole-cell currents were continuously recorded while bath [Na⁺] was rapidly increased from 1 mm (open bar) to 110 mm (gray bar).