FIGURE 4.

Overexpression of UTX promotes brown fat specific gene expression. A, UTX protein and H3K27me3 levels in control or overexpression cells with or without isoproterenol treatment. B–G, overexpression of UTX promotes the expression of brown fat genes including UCP1 (B), PGC1α (C), Acox1 (D), Dio2 (E), Elovl3 (F), and PGC1β (G). BAT1 cells were differentiated and then reversely transfected with UTX expression plasmids as described under “Experimental Procedures.” UTX and H3K27me3 protein levels were detected by immunoblotting and brown fat gene mRNA levels were measured by quantitative RT-PCR, as described in the “Experimental Procedures.” All data are expressed as mean ± S.E., n = 4; groups labeled with different letters are statistically different from each other. Isop: isoproterenol l (1 μm).