FIGURE 8.

UTX physically interacts with CBP in BAT1 cells. A, immunoprecipitation of UTX pulls down the CBP protein. B, immunoprecipitation of CBP pulls down the UTX protein. C, UTX and CBP have a weak interaction at endogenous levels. BAT1 cells were reversely transfected with UTX and CBP-HA expression plasmids as described in “Experimental Procedures.” Immunoprecipitation and immunoblotting were conducted as described under “Experimental Procedures.” For A and B, the whole cell lysates were used for IP and IB, and the antibody used for IP and IB CBP-HA was the antibody against HA. For C, the nuclear protein was extracted and used for IP and IB and the antibody used for IP and IB CBP-HA was the antibody against CBP. OE: overexpression.