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. 2015 Aug 21;290(41):25164–25173. doi: 10.1074/jbc.M115.665422

FIGURE 4.

FIGURE 4.

Potentiometric titrations of membranes containing variants of residues coordinating the distal pyranopterin of NarGHI. A, comparison of representative titrations of wild-type (■), S719A (▾), H1163A (▶), and H1184A (●) enzymes. Data were fit to the following parameters: wild-type, Em = 140 mV (E1 = 183 mV, E2 = 97 mV); S719A, Em = 150 mV (E1 = 176 mV, E2 = 124 mV); H1163A, Em = 58 mV (E1 = 49 mV, E2 = 68 mV); H1184A, Em = 103 mV (E1 = 96 mV, E2 = 111 mV). B, plot of residuals. C, summary of Em, E1, and E2 values for the variants based on the data presented in Table 1. Colored squares indicate the mean Em values and the horizontal lines indicate the E1 and E2 values in the cases where E1 − E2 > 0 (Kstab ≥ 1).