Skip to main content
. 2015 Aug 21;290(41):25164–25173. doi: 10.1074/jbc.M115.665422

FIGURE 5.

FIGURE 5.

Potentiometric titrations of membranes containing variants of His1092 and His1098. A, comparison of representative titrations of wild-type (■), H1092R (▾), H1098A (▶), and H1092A (●) enzymes. Data were fit to the following parameters: wild-type, Em = 140 mV (E1 = 183 mV, E2 = 97 mV); H1092R, Em = 155 mV (E1 = 213 mV, E2 = 97 mV); H1098A, Em = 35 mV (E1 = 129 mV, E2 = −60 mV); H1092A, Em = −2 mV (E1 = 24 mV, E2 = −29 mV). B, plot of residuals. C, summary of Em, E1, and E2 values for the variants based on the data presented in Table 1. Colored squares indicate the mean Em values and the horizontal lines indicate the E1 and E2 values.