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. 2015 Aug 31;290(41):25188–25196. doi: 10.1074/jbc.M115.667915

FIGURE 3.

FIGURE 3.

IL-1α binding to IL-1R2 prevents calpain cleavage and controls cytokine activity in ECs. A, RT-PCR for membrane (mIL-1R2) and soluble (sIL-1R2) IL-1R2 and GAPDH transcript within THP1 and HUVEC cells. B, dual immunofluorescence for pro-IL-1α and IL-1R2 in control HUVECs. Scale bars represent 50 μm. C, proximity ligation assay (DuoLink) showing association between IL-1α and IL-1R2 in control HUVEC cells. D, Western blot for IL-1R2 in whole cell lysates of HUVECs pretreated as indicated. E, mean fluorescent intensity (MFI) minus isotype control of HUVEC cells, pretreated as indicated, stained for IL-1R2, and analyzed by flow cytometry. Data represent the mean ± S.E. of n = 2.