FIGURE 4.

EC activation reduces the interaction between IL-1α and IL-1R2. A, Western blot for IL-1α in necrotic HUVEC lysates, pretreated as indicated, and incubated with increasing quantities of exogenous pro-IL-1α (p33). B, proximity ligation assay (DuoLink) showing association between IL-1α and IL-1R2 in HUVEC cells pretreated as indicated. C and D, relative number of interactions between IL-1α and IL-1R2 in HUVECs pretreated as indicated, assessed by DuoLink and quantified by blinded counting (C) and flow cytometry (D). MFI, mean fluorescent intensity. Data represent the mean ± S.E. of n = 2; *, p ≤ 0.05.