Figure 3. Neurons that elicit antennal grooming have neurites in the AMMC and/or SEZ.
(A–D) spGAL4 pairs targeting each neuronal class in the ventral brain: (A) aJO-spGAL4-1, (B) aBN1-spGAL4-1, (C) aBN2-spGAL4-2, and (D) aDN1-spGAL4-1. aDN1 is shown as an example in (D), but there are additional aDNs (aDN2 and aDN3, see Figure 2F and Figure 2—figure supplement 2G–I). CNSs stained with anti-GFP (green) and anti-bruchpilot (magenta). Maximum intensity projections are shown from frontal and lateral views. Arrows show the different projection regions: AMMC (white), posterior SEZ (yellow), and ventral SEZ (red). Scale bars, 25 μm. (E) Traced neurons in different colors manually aligned (also shown in Video 1).