FIG. 2.

HTNV A9 N protein differentially regulated the transcriptional activity of NFκB and IRF3. (A) Schematic picture of reporter plasmids used in our study. (B) HTNV A9 N protein (encoded by A9 S plasmid) inhibited RIG-I-induced NFκB activation in a dose-dependent manner. (C) HTNV A9 N protein enhanced RIG-I-induced IRF3 activation. The first two bars in (B, C) reflected the basal level of promoter activity (without overexpression of RIG-I). Results are expressed as mean±SEM. *p<0.05 and **p<0.01 (t-test). Representative results from at least three independent experiments. NFκB, nuclear factor kappa B.