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. 2015 Feb 9;2(1):e000018. doi: 10.1136/bmjgast-2014-000018

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Photomicrograph showing a biopsy of lung adenocarcinoma used as a positive control for inducible nitric oxide synthase (iNOS) immunostain. There is strong immunopositivity in the tumour cell nuclei and cytoplasm ((A) immunohistochemistry (IHC; iNOS) ×200). Photomicrograph of a biopsy showing baseline collagenous colitis; there is strong and diffuse immunopositivity for iNOS immunostain in surface and crypt mucosa. Few lymphocytes also showing positivity for iNOS ((B) IHC (iNOS) ×100). Eight weeks after VSL#3 treatment, there is a marked reduction in the area distribution and stain intensity of iNOS immunostain ((C) IHC (iNOS) ×40; Blue arrow showing nuclear positivity). Eighteen weeks post VSL#3 treatment, there is still a very minimum expression of iNOS in the colonic biopsy ((D) IHC (iNOS) ×40; Brown arrow showing occasional nuclear positivity).