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. 2015 Apr 17;2(1):e000022. doi: 10.1136/bmjgast-2014-000022

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Experimental workflow. The figure summarises the workflow used in this study. Participants recruited had biopsies taken and provided stool (for faecal butyrate) and diet information. The frozen biopsies were lysed to yield soluble and insoluble fractions. The former was pooled according to butyrate and subject to immunoprecipitation for acetyl proteins, to yield an acetyl-enriched and a soluble fraction. The former was analysed by 2DGE whereas the latter was analysed by iTRAQ. The insoluble fraction was processed to yield IF and then analysed by iTRAQ. iTRAQ, isobaric tags for relative and absolute quantification; 2DGE, two dimensional gel electrophoresis; IF, intermediate filament.