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. 2015 Sep 7;47(5):1672–1684. doi: 10.3892/ijo.2015.3147

Figure 7.

Figure 7

Sox4 is a direct target of miR-132. (A) The protein expression of Sox4 was determined by western blotting in SaOS-2 and 143B cells transfected with miR-132 mimic or miR-NC. α-tubulin was detected as a loading control. (B) The protein expression of Sox4 was determined by western blotting in SaOS-2 and 143B cells transfected with miR-132 inhibitor or anti-miR-NC. (C) Schematic representation of Sox4 3′UTRs showing putative miRNA target site. (D) The analysis of the relative luciferase activities of Sox4-WT, Sox4-MUT in osteosarcoma cells. All data are presented as mean ± SEM, n=6. ##P<0.01 vs. miR-NC or anti-miR-NC.