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. 2015 Jun 28;6(18):15842–15856. doi: 10.18632/oncotarget.4683

Figure 1. Regulation of CST5 by p53 in SW480 colorectal cancer cells.

Figure 1

SW480 cells harboring a pRTR-p53-VSV vector or the empty vector pRTR were treated with DOX for the indicated periods of time to activate p53-VSV expression. A. Western blot analysis of the indicated proteins in SW480/pRTR-p53-VSV. β-actin served as a loading control. B. The expression of the CST5- and p21-mRNA was determined by qPCR analysis. Fold changes represent mean values of triplicate analyses of DOX-versus un-treated cells normalized to β-actin expression. Standard deviations are represented by error bars. C. HEK293T cells were transfected with the pRTR-p53-VSV vector and p53 expression was induced by adding DOX for 24 hours. The expression of CST5- and p21-mRNA levels was measured by qPCR analysis. Fold changes represent mean values of triplicate analyses of DOX-treatment versus untreated cells normalized to β-actin expression. Standard deviations are represented by error bars. D. Analysis of CST5 expression and localization in SW480/pRTR-p53-VSV cells by confocal immunofluorescence microscopy after 48 hours with and without DOX treatment. Nuclear DNA was visualized by DAPI staining. 200x magnification.