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. 2015 Jun 28;6(18):15842–15856. doi: 10.18632/oncotarget.4683

Figure 4. CST5 is relevant for p53-mediated MET.

Figure 4

SW480/pRTR-p53-VSV cells were transfected with a CST5-specific siRNA or control oligonucleotide for 72 hours and treated with DOX for 48 hours. A. Western Blot analysis of the indicated proteins. β-actin served as a loading control. B. SNAIL mRNA levels were measured by qPCR analysis after treatment with DOX. Fold changes represent mean values of triplicate analyses of DOX treatment versus vehicle treated cells normalized to β-actin expression. The relative p53-mediated repression of SNAIL is indicated. C., D. Wound-healing assay using Ibidi-Inlets. C. Representative pictures of the wound area obtained 48 hours after scratching. 100 x magnification. D. Left: Average [%] of the closed wound area determined by the final width of the scratch in three independent wells. Right: The relative p53-mediated inhibition of wound-closure. E. Boyden chamber-assay of cellular migration. Left: The relative migration through the filter with the untreated control set as one. Right: The relative p53-mediated inhibition of migration. B, D, E: Error bars represent +/− SD (n = 3).