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. 2015 Jun 28;6(18):15842–15856. doi: 10.18632/oncotarget.4683

Figure 6. Calcitriol treatment prevents SNAIL-mediated repression of CST5.

Figure 6

A. CST5 and SNAIL mRNA levels were measured by qPCR analysis after treatment with DOX at the indicated time-points in SW480/pRTR-SNAIL-VSV cells. Fold changes represent mean values of triplicate analyses after DOX treatment versus vehicle treated cells normalized to β-actin expression. B. Schematic SNAIL binding site 50 bp upstream of the CST5 transcriptional start site. C. qChIP analysis of DLD1/pRTR-SNAIL-VSV cells 24 hours after activation of SNAIL-VSV expression by addition of DOX using anti-VSV and anti-rabbit IgG for ChIP; this ChIP analysis was performed in unicates and measured in triplicates. D. CST5 and SNAIL mRNA levels were measured by qPCR analysis after treatment with DOX, calcitriol or both agents at the indicated time-points in SW480/pRTR-SNAIL-VSV cells. Fold changes represent mean values of triplicate analyses of DOX / calcitriol / DOX+calcitriol treatment versus vehicle treated cells normalized to β-actin expression. Error bars represent standard deviations.