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. 2015 May 8;6(18):15891–15901. doi: 10.18632/oncotarget.4045

Figure 1. Plzf expression is elevated in aged mouse testes.

Figure 1

A, qPCR analysis of Plzf mRNA in young and aged testes normalized to Ddx4. Graphs represent mean value ± s.e.m. n = 9 mice per group. *P < 0.05. B, representative images of IHC for Plzf in young and aged testes. Images are representative of testes from 3 mice examined. Arrow, low Plzf. Arrowhead, high Plzf. *, degenerative seminiferous tubule. C, representative flow profile of detecting Plzf-expressing cells by intracellular staining and FACS by using anti-Plzf antibody (PE-conjugated). D, quantification of the percentage of Plzf-positive cells analyzed by FACS in panel C. Graphs represent mean value ± s.e.m. n = 6 mice per group. *P < 0.05. E, Plzf intensity in Plzf-expressing cells from young versus aged mice. F, Quantification of Plzf expression levels in young and aged testes. Graphs represent mean value ± s.e.m. n = 4 mice per group. *P < 0.05.