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. 2015 Apr 3;6(18):15940–15952. doi: 10.18632/oncotarget.3612

Figure 3. F806 inhibits cell adhesions and induces anoikis in ESCC cells.

Figure 3

A. EC109 and KYSE510 cells were suspension cultured on poly-HEMA-coated plates in the presence of F806 for 48 h and anoikis ratio was then determined by MTT assay. B. EC109 and KYSE510 cells were kept in fibronectin (FN), collagen (COL) or laminin (LAM)-coated plates in the presence of F806 for 24 h. C. EC109 and KYSE510 cells were kept in FN or COL-coated plates for 30 min and treated with F806 for another 24 h. D. EC109 and KYSE510 cells were kept in FN or COL-coated plates for 24 h prior to 30 min of F806 stimulation. The attached cells were recorded by MTT assay in each group. Data represent three independent experiments; *P < 0.05 vs. control group; mean ± SD, n = 3–6.