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. 2015 Apr 18;6(18):15984–15994. doi: 10.18632/oncotarget.3762

Figure 3. MiR-622 targets directly the RB1.

Figure 3

A. Schematic illustration of the potential miR-622-binding sites in 3′-UTRs of E2F8, E2F1, and RB1. B. Luciferase activity of 293FT cells transfected with luciferase vectors containing wild-type or mutant plasmids and mimics-NC (0.5 uM) or mimics-miR-622 (0.5 uM), respectively. C. Luciferase assay of SW837 cells transfected with the indicated reporters and mimics-miR-622 or mimics-control. D. Folds change of RB1 in mRNA level in cells transfected with lentivirus-miR-622 and lentivirus-miR-control. E. Rb expression variation of cells transfected by lentivirus-miR-622 and lentivirus-miR-control analyzed by Western blotting. F. Rb and E2F1 variation in response to radiation. RNU6B and GAPDH were internal controls of miR-622 and RB1 for qRT-PCR. GAPDH was an internal control of Western blotting.