Figure 4. The role of Caspase-3 in the cell death induced by MV-Edm and the MV-Edm replication.

a. At indicated times, the cell viability of each group was assessed with MTS assay. b. The intracellular virus replication of SiHa cells, expressed as TCID₅₀/ml, was determined at different times after infection at an MOI of 1. c. The viral mRNA levels were measured using real-time PCR. Each value is normalized to that in SiHa cells transfected with empty vector (SiHa^sh-cont), which was set a ratio = 1, and represented with mean ± SD. d. The comparisons of virus production and cellular apoptosis induced by MV-Edm in SiHa cells from each group. e. Liner Fit curve between cellular apoptosis and virus production. *means P < 0.05.