Skip to main content
. 2015 Apr 19;6(18):16069–16083. doi: 10.18632/oncotarget.3866

Figure 2. CaMKIIγ is required for maintenance of stem-like and tumorigenic properties.

Figure 2

Figure 2

A. Relative gene expression of CaMKIIγ in indicated parental or oncosphere cells by real-time PCR. Data are expressed as mean ± SEM of n = 3 independent cell dished per condition. *p < 0.05, **p < 0.01, ***p < 0.001 versus parental cells. B. Detection of activated (Phosphorylation of Ser287) and total CaMKIIγ protein level by western blots in parental or oncosphere cells from lung cancer cells (A549, H1299 and HCC827), primary lung cancer cells (ZRLC-1, ZRLC-3 and ZRLC-5) or primary normal lung cells (ZRNL-4, ZRNL-18 and ZRNL-19). C. Detection of activated and total CaMKIIγ protein level by western blots in sorted lung cancer cells. Quantitative analysis of oncosphere formation by 1000 control (shCtl) and CaMKIIγ knockdown (shCaM) ZRLC-1 or HCC827 cells. Data are expressed as mean ± SEM of n = 3 independent cell dishes per condition. *p < 0.05, **p < 0.01, ***p < 0.001 versus shCtl cells. D. Relative gene expression of OCT4, NANOG, MYC, and KLF4 in shCtl and shCaM ZRLC-1 or HCC827 cells by real-time PCR. Data are expressed as mean ± SEM of n = 3 independent cell dishes per condition. *p < 0.05, **p < 0.01, ***p < 0.001 versus shCtl cells. E. Detection of Oct4, c-Myc, CaMKIIγ, and GAPDH protein by western blots in shCtl and shCaM ZRLC-1 or HCC827 cells. F. ShCtl and shCaM ZRLC-1 or HCC827 cells were separately injected subcutaneously into nude mice. Data are expressed as mean ± SEM of n = 5 mice per group. *p < 0.05, **p < 0.01. NS: no significance. Tumor incidence is displayed on the graph. G. TIC frequency of shCtl and shCaM ZRLC-1 or HCC827 cells is measured by LDA in vivo.