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. 2015 Apr 15;6(18):16239–16252. doi: 10.18632/oncotarget.3842

Figure 1. Establishment of ING5 overexpression and knockdown cell lines.

Figure 1

A. mRNA expression level of ING5 in lung cancer cell lines and normal human bronchial epithelial (HBE) cells by qRT-PCR. Data are shown as mean plus standard error of three independent experiments. B. Analysis of protein levels of ING5 in lung cancer cell lines and normal HBE cells by western blot. C. ING5-overexpressing A549 cells were generated using a lentiviral system. Empty vector-infected cells were used as control. ING5 protein levels in A549 control and A549 ING5 overexpression (ING5) cells were detected by western blot. Actin was used as an internal loading control. D. Stably ING5-depleted H1299 cells were generated using shING5-containing viruses. Cells infected with vector-scramble sequence were used as control (shControl). ING5 protein levels in H1299 shControl and H1299 ING5 knockdown (shING5) cells were determined by western blot.