Figure 6. Ericifolin is not the active component in AAE against breast cancer.
A. Cytotoxicity profile of different AAE fractions tested against LNCaP prostate cancer cells and MCF7 cells. A 48-well plate was seeded with 5 × 103 cells/well and treated with AAE fractions (100 μg/ml) for 72 h and cell viability was estimated by MTT assay. B. & C. The cytotoxicity of fraction-174 (Ericifolin purified from AAE by an independent strategy) was tested in both B. MCF7 and C. MB231 cells using MTT assay. D. Ericifolin does not induce apoptosis or autophagy in MDA-MB231 cells. 10,000 cells were plated in 24-well clusters and treated with Ericifolin (50 μg/ml) for 24 h. Cell lysates were made and tested against c-PARP and LC3B for apoptosis and autophagy activity, respectively.