Fig. 6.

Immunostaining for RIP3 using propidium iodide (PI)-labeled sections obtained 3 days after hemisection. The representative pictures show that the PI-labeled cells were frequently observed to be RIP3-positive (a–c). At higher magnification (d–f), most of the nuclei in the PI-labeled cells expressing RIP3 (arrowheads in f) were round, which is normally observed in cells undergoing necrotic cell death, not fragmented or shrunken, as observed in apoptotic cells. Scale bars 100 μm (a–c), 20 μm (d–f). The schematic drawing illustrates the location of the micrographs (g)