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. 2015 May 14;4(5):e001986. doi: 10.1161/JAHA.115.001986

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© 2015 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Activity of NRF2 in skeletal muscle of CLI patients. A, Represents mRNA expression of NRF2 in the skeletal muscle of control and CLI patients. B, Represents immunoblots of nuclear levels of NRF2 and fibrillarin in the skeletal muscle of control and CLI patients. C, Quantitation of immunoblots in (B). D, NRF2-DNA binding activity as judged by EMSA; lanes 1 and 2, control; lanes 3 and 4, CLI. E, The binding specificity of NRF2; lane 1, free probe; lane 2, NE; lane 3, NE+W/T oligo (250× excess); lane 4, NE+NRF2 antibody; and lane 5, NE+nonspecific antibody (non sp. ab). Circle with number inside bar denotes number of patients per group. CLI indicates critical limb ischemia; EMSA, electrophoretic mobility shift assay; NE, nuclear extracts; NRF2, nuclear factor-erythroid 2-related factor 2; W/T, wild type.