Table 2.
Open reading frames encoding multidomain NRPSs and PKSs in modular NRPS and PKS gene clusters of Herbidospora strains
| Gene cluster | Scaffold-orf no. (size, % identity/similarity to closest homolog) | Domain organization | Presumed product | Closest homolog | |||||
|---|---|---|---|---|---|---|---|---|---|
| H. cretacea NBRC 15474T | H. mongoliensis NBRC 105882T | H. yilanensis NBRC 106371T | H. daliensis NBRC 106372T | H. sakaeratensis NBRC 102641T | Accession no. | Origin | |||
| nrps-1 | s01-orf204 (6699aa, 88/92) | s02-orf3 (6749aa, 85/90) | s34-orf56 (6711aa, 92/94) | s03-orf732 (6697aa, 89/93) | s01-orf733 (6657aa, 91/94) | A/MT/T-C/Aser/T-C/Aorn/MT/T-C/A/T-C/Aser/T-C/A/T/E | x-Ser-mOrn-x-Ser-x (albachelin-like siderophore) | WP_034384656 | Herbidospora cretacea NRRL B-16917 |
| nrps-2 | s17-orf53* (1026aa, 91/93) | s03-orf338* (988aa, 89/92) | s13-orf130* (1010aa, 93/94) | s05-orf276* (1051aa, 91/94) | s15-orf51* (974aa, 91/94) | A/T-C | x-Gly-Lys-x | WP_030456013 | Herbidospora cretacea NRRL B-16917 |
| s17-orf54 (973aa, 88/92) | s03-orf339 (990aa, 86/91) | s13-orf129 (1015aa, 90/92) | s05-orf275 (974aa, 90/93) | s15-orf52 (975aa, 91/94) | C/Agly/T | WP_030456012 | Herbidospora cretacea NRRL B-16917 | ||
| s17-orf55 (979aa, 88/90) | s03-orf340 (991aa, 85/89) | s13-orf128 (972aa, 90/92) | s05-orf274 (961aa, 91/93) | s15-orf53 (961aa, 91/93) | C/A(lys)/T | WP_034385955 | Herbidospora cretacea NRRL B-16917 | ||
| s17-orf58 (1351aa, 88/92) | s03-orf343 (1341aa, 89/93) | s13-orf125 (1348aa, 94/96) | s05-orf271 (1343aa, 92/95) | s15-orf56 (1350aa, 92/95) | C/A/T-TE | WP_030456008 | Herbidospora cretacea NRRL B-16917 | ||
| nrps-3 | s08-orf344 (2105aa, 86/90) | s17-orf93 (2104aa, 85/90) | s12-orf143 (2199aa, 90/91) | s01-orf374 (2125aa, 89/92) | s17-orf87 (2150aa, 90/92) | Agly/T-C/T-C/Agly/T | Gly-?-Gly-Asp | WP_034384991 | Herbidospora cretacea NRRL B-16917 |
| s08-orf343 (1775aa, 91/95) | s17-orf92 (1766aa, 86/92) | s12-orf144 (1766aa, 95/97) | s01-orf373 (1776aa, 92/96) | s17-orf86 (1777aa, 92/95) | C/Aasp/T-TE | WP_030453964 | Herbidospora cretacea NRRL B-16917 | ||
| nrps-4 | s01-orf4* (1934aa, 88/91) | s02-orf218* (1932aa, 88/93) | s08-orf109* (1933aa, 95/97) | s03-orf522* (1932aa, 93/95) | s01-orf545* (1932aa, 94/95) | C/A/T-C/Alys/T | x-Lys-Asp | WP_030453192 | Herbidospora cretacea NRRL B-16917 |
| s01-orf5 (1721aa, 93/96) | s02-orf217 (1722aa, 90/94) | s08-orf108 (1721aa, 96/98) | s03-orf523 (1721aa, 94/96) | s01-orf546 (1721aa, 95/96) | C/Aasp/T-TE | WP_030453193 | Herbidospora cretacea NRRL B-16917 | ||
| nrps-5 | s03-orf142 (1742aa, 87/91) | s04-orf515 (1751aa, 86/91) | s21-orf82 (1738aa, 95/97) | s01-orf929 (1731aa, 91/93) | s02-orf479 (1729aa, 91/94) | C/Aasp/T-TE | Gly-Asp | WP_030454483 | Herbidospora cretacea NRRL B-16917 |
| s03-orf139 (1011aa, 93/95) | s04-orf518 (1011aa, 93/95) | s21-orf85 (1011aa, 96/97) | s01-orf932 (1011aa, 93/96) | s02-orf482 (1011aa, 94/96) | C/Agly/T | WP_034384898 | Herbidospora cretacea NRRL B-16917 | ||
| nrps-6 | s06-orf42* (474aa, 87/91) | s05-orf174* (470aa, 86/90) | s06-orf123* (471aa, 92/94) | s14-orf189* (471aa, 92/93) | s12-orf45* (471aa, 92/94) | C/T | ?-Asn | WP_030450820 | Herbidospora cretacea NRRL B-16917 |
| s06-orf40 (1678aa, 57/68) | s05-orf172 (1716aa, 55/66) | s06-orf125 (1684aa, 57/68) | s14-orf187 (1677aa, 57/67) | s12-orf47 (1677aa, 57/67) | C/Aasn/T-TE | WP_031172905 | Streptosporangium roseum NRRL B-2638 | ||
| nrps-7 | s05-orf29 (1709aa, 91/94) | s02-orf251 (1777aa, 90/94) | s08-orf140 (1776aa, 96/97) | s03-orf492 (1789aa, 94/96) | s01-orf513 (1812aa, 94/95) | C/Aasn/T-TE | (Asn) | WP_030453154 | Herbidospora cretacea NRRL B-16917 |
| nrps-8 | – | – | s05-orf295 (1018aa, 92/94) | s03-orf778 (1021aa, 89/92) | s01-orf772 (1019aa, 89/91) | A/T-TE | x–x | WP_030453437 | Herbidospora cretacea NRRL B-16917 |
| s05-orf293 (1097aa, 95/97) | s03-orf780 (1097aa, 93/96) | s01-orf774 (1097aa, 94/96) | C/A/T | WP_030453439 | Herbidospora cretacea NRRL B-16917 | ||||
| nrps-9 | – | – | s02-orf346 (3681aa, 56/63) | – | s02-orf28 (3673aa, 53/63) | Agly/T-C/Aasn/T-C/A/T-C/A(lys)/T | Gly-Asn-x-Lys-Thr-x | WP_026126874 | Nocardiopsis xinjiangensis YIM 90004 |
| s02-orf347 (2407aa, 59/70) | s02-orf27 (2385aa, 59/70) | C/Athr/T-C/A/T-TE | WP_040918882 | Saccharomonospora glauca K62 | |||||
| nrps-10 | s05-orf197* (1110aa, 58/67) | s02-orf401* (1110aa, 58/68) | – | – | – | T-C/Aser/T | x-Ser-?-x–x-Ser | WP_037800301 | Streptomyces sp. Mg1 |
| s05-orf195* (1679aa, 37/48) | s02-orf399* (1679aa, 37/48) | C/T-C/A/T | AGC43421 | Myxococcus stipitatus DSM 14675 | |||||
| s05-orf184 (540aa, 39/52) | s02-orf388 (527aa, 38/50) | A/T | WP_017558240 | Nocardiopsis baichengensis YIM 90130 | |||||
| s05-orf182 (1693aa, 48/57) | s02-orf386 (1700aa, 46/57) | C/T-C/Aser/T-TE | ACU38342 | Actinosynnema mirum DSM 43827 | |||||
| nrps-11 | s08-orf166 (1237aa, 63/71) | s13-orf80 (1230aa, 65/73) | – | – | – | C/A/T-TE | x | ACU75141 | Catenulispora acidiphila DSM 44928 |
| nrps - 12 | – | – | – | s01-orf155 (3126aa, 96/97) | – | C/Aphe/T-C/Aasp/T-C/Aasp/T | Phe-Asp-Asp-x-Asp-Ser-x–x-Phe-x-Val-x-Tyr-Asp-Asn-Tyr-x-Tyr-Asp–Asp-x-Asp | WP_034385663 | Herbidospora cretacea NRRL B-16917 |
| s01-orf140 (11331aa, 55/65) | – | C/A/T-C/Aasp/T-C/Aser/T-C/A/T-C/A/T-C/Aphe/T-C/A/T-C/Aval/T-C/A/T-C/Atyr/T-C/Aasp/T | EIF87998 | Streptomyces tsukubensis NRRL 18488 | |||||
| s01-orf139 (8503aa, 54/64) | – | C/Aasn/T-C/Atyr/T-C/A/T-C/Atyr/T-C/Aasp/T-C/Aasp/T-C/A/T-C/Aasp/T-TE | EIF87998 | Streptomyces tsukubensis NRRL 18488 | |||||
| nrps - 13 | – | – | – | s02-orf353 (1637aa, 51/59) | – | C/Agly-C/Aala/T | Gly-Ala | WP_026213748 | Nonomuraea coxensis DSM 45129 |
| nrps - 14 | – | – | – | – | s01-orf286 (2965aa, 39/50) | C/A/T-C/A/T-C/Aser/T | Val-x-x-Ser-Asn–Asn | ERK92233 | Myxococcus sp. (contaminant ex DSM 436) |
| s01-orf285 (2462aa, 56/67) | C/Aasn/T-C/Aasn/T-C | WP_039739900 | Saccharomonospora halophila 8 | ||||||
| s01-orf277* (620aa, 52/63) | Aval/T | WP_033666206 | Salinispora pacifica CNS055 | ||||||
| nrps - 15 | – | – | – | – | s13-orf204 (1086aa, 58/66) | Agly/C/T | ?-Gly | ETK35217 | Microbispora sp. ATCC PTA-5024 |
| nrps - 16 | – | – | – | – | s14-orf131 (1233aa, 66/75) | C/A/T | x | ELS56639 | Streptomyces viridochromogenes Tue57 |
| pks/nrps-1 | s04-orf226 (3534aa, 82/87) | s07-orf8 (3458aa, 82/87) | s05-orf58 (3534aa, 90/92) | s06-orf132 (3570aa, 84/88) | s01-orf1038 (3515aa, 86/89) | CoL/ACP-KS/AT/KR/ACP-C/A/T-C | ?-pk-x | WP_034384725 | Herbidospora cretacea NRRL B-16917 |
| pks/nrps - 2 | – | s11-orf48 (1776aa, 52/66) | – | – | – | KS/AT/DH/KR/ACP | pk-x-x–x-Val-x-x-Ser-Thr-Asn-Asn–Asn-Thr-Asn | EPH45771 | Streptomyces aurantiacus JA 4570 |
| s11-orf45 (1637aa, 50/59) | A/T-C/A/T | EPH45774 | Streptomyces aurantiacus JA 4570 | ||||||
| s11-orf44 (1073aa, 71/76) | C/A/T | CAC01623 | Planobispora rosea ATCC 53733 | ||||||
| s11-orf43 (2007aa, 50/60) | C/Aval/T-C/A/T | EPH43046 | Streptomyces aurantiacus JA 4570 | ||||||
| s11-orf41 (2145aa, 47/57) | C/A/T-C/Aser/T | EPH43048 | Streptomyces aurantiacus JA 4570 | ||||||
| s11-orf40 (3193aa, 41/54) | C/Athr/T-C/Aasn/T-C/Aasn/T | WP_018350842 | Longispora albida DSM 44784 | ||||||
| s11-orf39 (1064aa, 45/58) | C/Aasn/T | WP_030327232 | Streptomyces sp. NRRL B-3229 | ||||||
| s11-orf38 (2516aa, 44/55) | C/Athr/T-C/Aasn/T-TE | EPH43049 | Streptomyces aurantiacus JA 4570 | ||||||
| pks/nrps - 3 | – | – | s05-orf134 (1316aa, 44/56) | – | – | KS/AT/ACP-TE | Leu-Val-Leu-Ser-pk | EWC62839 | Actinokineospora sp. EG49 |
| s05-orf130 (1006aa, 42/59) | Aleu/T/E | BAH43926 | Brevibacillus brevis NBRC 100599 | ||||||
| s05-orf129 (3118aa, 46/60) | C/Aval/T-C/Aleu/T-C/Aser/T | AGC43421 | Myxococcus stipitatus DSM 14675 | ||||||
| pks/nrps - 4 | – | – | – | – | s08-orf1 (> 346aa, 55/61) | …ACP | See Fig. 1h | WP_037075741 | Pseudonocardia spinosispora DSM 44797 |
| s08-orf2 (6590aa, 72/79) | KS/ATm/DH/KR/ACP-KS/ATm/DH/KR/ACP-KS/ATm/DH/KR/ACP-KS/AT/DH | WP_042407435 | Streptacidiphilus carbonis NBRC 100919 | ||||||
| s08-orf2_1 (379aa, 83/88) | KR/ACP | WP_042407435 | Streptacidiphilus carbonis NBRC 100919 | ||||||
| s08-orf3 (4716aa, 57/67) | KS/AT/KR/ACP-KS/AT/KR/ACP-KS/AT/KR/ACP-TE | WP_037075679 | Pseudonocardia spinosispora DSM 44797 | ||||||
| s08-orf8 (7045aa, 69/77) | KS/ATm/ACP-KS/AT/DH/ER/KR/ACP-KS/AT/DH/ER/KR/ACP-KS/AT/DH/ER/KR/ACP | WP_037075740 | Pseudonocardia spinosispora DSM 44797 | ||||||
| s08-orf9 (5636aa, 51/62) | KS/AT/DH/ER/KR/ACP-KS/AT/DH/ER/KR/ACP-KS/AT/DH/KR/ACP | ADL46003 | Micromonospora aurantiaca ATCC 27029 | ||||||
| s08-orf10* (997aa, 65/76) | A/T | WP_042397191 | Streptacidiphilus carbonis NBRC 100919 | ||||||
| pks-1 | s14-orf123 (2788aa, 90/92) | s10-orf265 (2815aa, 89/92) | s18-orf5 (2796aa, 90/92) | s03-orf254 (2800aa, 89/91) | s01-orf250 (2782aa, 91/94) | KS/ATm/ACP-KS/ATm/DH/KR/ACP | See Fig. 1a | WP_034382778 | Herbidospora cretacea NRRL B-16917 |
| s14-orf124 (1807aa, 93/95) | s10-orf264 (1806aa, 93/96) | s18-orf6 (1800aa, 94/96) | s03-orf255 (1794aa, 94/96) | s01-orf251 (1793aa, 94/95) | KS/AT/DH/KR/ACP | WP_030450097 | Herbidospora cretacea NRRL B-16917 | ||
| s14-orf125 (1800aa, 91/93) | s10-orf263 (1801aa, 92/93) | s18-orf7 (1795aa, 92/94) | s03-orf256 (1795aa, 90/93) | s01-orf252 (1798aa, 92/93) | KS/ATm/DH/KR/ACP | WP_030450096 | Herbidospora cretacea NRRL B-16917 | ||
| s14-orf126 (1567aa, 91/94) | s10-orf262 (1578aa, 92/94) | s18-orf8 (1573aa, 91/94) | s03-orf257 (1567aa, 91/94) | s01-orf253 (1595aa, 90/92) | KS/AT/KR/ACP | WP_030450095 | Herbidospora cretacea NRRL B-16917 | ||
| s14-orf127 (3057aa, 92/)94 | s10-orf261 (3070aa, 91/94) | s18-orf9 (3062aa, 92/94) | s03-orf258 (3031aa, 92/94) | s01-orf254 (3033aa, 92/94) | KS/AT/KR/ACP-KS/AT/KR/ACP | WP_030450094 | Herbidospora cretacea NRRL B-16917 | ||
| s14-orf135* (1265aa, 94/95) | s10-orf253* (1264aa, 95/96) | s18-orf17* (1261aa, 94/96) | s03-orf266* (1264aa, 94/96) | s01-orf262* (1261aa, 95/96) | KS/KR/ACP | WP_034382545 | Herbidospora cretacea NRRL B-16917 | ||
| s14-orf136* (1573aa, 95/97) | s10-orf252* (1575aa, 95/97) | s18-orf18* (1573aa, 95/97) | s03-orf267* (1573aa, 94/96) | s01-orf263* (1567aa, 96/97) | KS/AT/KR/ACP | WP_034382775 | Herbidospora cretacea NRRL B-16917 | ||
| pks-2 | – | s04-orf63 (6103aa, 52/62) | s12-orf1 (> 2049aa, 54/63) | s01-orf485 (6202aa, 50/60) | s26-orf2 (5982aa, 56/65) | (KS)/AT/ACP-KS/AT/DH/KR/ACP-KS/AT/DH/KR/ACP-KS/AT/DH/KR/ACP | See Fig. 1b | AEB44393 | Verrucosispora maris AB-18-032 |
| s47-orf2 (> 3456aa, 53/62) | |||||||||
| s04-orf64 (3854aa, 58/66) | s47-orf1 (> 566aa, 71/78) | s01-orf486 (3774aa, 57/64) | s26-orf1 (> 1579aa, 53/60) | KS/AT(m,e)/DH/KR/ACP-KS/AT(m)/DH/ER/KR/ACP | ABW12874 | Frankia sp. EAN1pec | |||
| s02-orf383 (> 1384aa, 66/72) | s02-orf1 (> 2192aa, 63/70) | ||||||||
| s04-orf65 (1070aa, 81/86) | s02-orf382 (997aa, 91/93) | s01-orf487 (1019aa, 87/90) | s02-orf2 (996aa, 89/91) | KS/AT/ACP | WP_030454081 | Herbidospora cretacea NRRL B-16917 | |||
| pks-3 | s12-orf80 (1259aa, 88/92) | – | s09-orf200 (1258aa, 95/96) | s01-orf1205 (1258aa, 94/96) | – | KS/AT?/DH/ACP | ? | WP_030454772 | Herbidospora cretacea NRRL B-16917 |
| pks-4 | – | – | s27-orf91 | s06-orf349 (1795aa, 53/64) | – | KS/AT/ACP/KR/DH | Enediyne | AAP92148 | Actinomadura verrucosospora ATCC 39334 |
| pks-5 | s04-orf1 (> 901aa, 70/79) | s28-orf45 (> 660aa, 64/73) | – | – | – | KS/AT… | See Fig. 1c | WP_018514184 | Streptomyces sp. ScaeMP-e10 |
| s36-orf55 (> 3542aa, 58/67) | s11-orf1 (> 405aa, 54/66) | …ACP-KS/AT/DH/KR/ACP-KS/AT/DH/KR/ACP | WP_032771883 | Streptomyces cyaneofuscatus NRRL B-2570 | |||||
| s11-orf2 (3315aa, 50/61) | |||||||||
| s36-orf54 (2142aa, 66/75) | s11-orf3 (2345aa, 50/62) | KS/AT(m)/DH/KR/ACP | WP_032771870 | Streptomyces cyaneofuscatus NRRL B-2570 | |||||
| pks - 6 | s01-orf132 (1020aa, 52/63) | – | – | – | – | KS/ATm/ACP | See Fig. 1d | EJJ02887 | Streptomyces auratus AGR0001 |
| s01-orf133 (7608aa, 50/61) | KS/AT/KR/ACP-KS/AT/DH/ER/KR/ACP-KS/AT/KR/ACP-KS/ATm/KR/ACP-KS/AT/KR/ACP | WP_035304435 | Actinokineospora inagensis DSM 44258 | ||||||
| s01-orf134 (4820aa, 54/64) | KS/AT/KR/ACP-KS/AT/DH/ER/KR/ACP-KS/AT/KR/ACP | EHY88974 | Saccharomonospora azurea NA-128 | ||||||
| s01-orf135 (6313aa, 52/63) | KS/ATm/KR/ACP-KS/AT/DH/KR/ACP-KS/AT/DH/KR/ACP-KS/AT/DH/KR/ACP | ACU36619 | Actinosynnema mirum DSM 43827 | ||||||
| s01-orf136 (4533aa, 55/65) | KS/AT/DH/KR/ACP-KS/AT/KR/ACP-KS/AT/KR/ACP | AAX98184 | Streptomyces aizunensis NRRL B-11277 | ||||||
| s01-orf137 (4803aa, 55/65) | KS/ATe(m)/DH/KR/ACP-KS/ATe(m)/KR/ACP-KS/AT/DH/KR/ACP | WP_033261216 | Amycolatopsis vancoresmycina NRRL B-24208 | ||||||
| s01-orf138 (5850aa, 54/65) | KS/AT/KR/ACP-KS/ATm/KR/ACP-KS/ATm/KR/ACP-KS/AT/KR/ACP | ABC87511 | Streptomyces sp. NRRL 30748 | ||||||
| s01-orf139 (4995aa, 52/64) | KS/ATm/KR/ACP-KS/AT/DH/ER/KR/ACP-KS/AT/DH/KR/ACP | AHH99925 | Kutzneria albida DSM 43870 | ||||||
| s01-orf140 (5337aa, 54/64) | KS/AT/KR/ACP-KS/AT/DH/ER/KR/ACP-KS/AT/DH/ER/KR/ACP | AEP40936 | Nocardiopsis sp. FU40 | ||||||
| s01-orf141 (3460aa, 57/68) | KS/AT/DH/KR/ACP-KS/AT/DH/KR/ACP | WP_035796302 | Kitasatospora mediocidica KCTC 9733 | ||||||
| s01-orf142 (4383aa, 54/65) | KS/AT/KR/ACP-KS/ATm/KR/ACP-KS/AT/KR/ACP | AAX98186 | Streptomyces aizunensis NRRL B-11277 | ||||||
| s01-orf143 (1247aa, 53/64) | KS/AT/ACP-TE | KIR65900 | Micromonospora carbonacea JXNU-1 | ||||||
| pks - 7 | s20-orf48 (2065aa, 59/68) | – | – | – | – | KS/ATe(m)/ACP-KS/AT/DH/ACP | See Fig. 1e | WP_042439448 | Streptacidiphilus albus NBRC 100918 |
| s20-orf49 (1530aa, 53/63) | KS/AT/KR/ACP | WP_042494385 | Streptomyces avermitilis MA-4680 = NBRC 14893 | ||||||
| s20-orf50 (2820aa, 62/70) | KS/AT/DH/KR/ACP-KS/ATm/ACP | CCH32016 | Saccharothrix espanaensis DSM 44229 | ||||||
| s20-orf51 (2064aa, 57/66) | KS/AT/DH/KR/ACP-TE | WP_041313683 | Saccharothrix espanaensis DSM 44229 | ||||||
| pks - 8 | – | s08-orf244 (5375aa, 59/69) | – | – | – | KS/AT/DH/ER/KR/ACP-KS/ATe(m)/KR/ACP-KS/ATm/DH/KR/ACP | See Fig. 1f | WP_033660827 | Salinispora pacifica CNS237 |
| s08-orf245 (1559aa, 55/66) | KS/AT/KR/ACP | EXU62139 | Streptomyces sp. PRh5 | ||||||
| s08-orf246 (3222aa, 53/65) | KS/ATe(m)/KR/ACP-KS/AT/DH/KR/ACP | EGX61520 | Streptomyces zinciresistens K42 | ||||||
| s08-orf251 (10965aa, 51/63) | KS/AT/ACP-KS/ATe(m)/DH/KR/ACP-KS/ATm/KR/ACP-KS/AT/DH/KR/ACP-KS/ATe(m)/DH/KR/ACP-KS/ATe(m)/KR/ACP-KS/AT/KR/ACP | ADI03772 | Streptomyces bingchenggensis BCW-1 | ||||||
| s08-orf252 (1565aa, 64/73) | KS/ATm/KR/ACP | WP_033775512 | Salinispora pacifica DSM 45546 | ||||||
| s08-orf253 (3638aa, 59/69) | KS/AT/DH/ER/KR/ACP-KS/ATm/KR/ACP | CAJ88176 | Streptomyces ambofaciens ATCC 23877 | ||||||
| s08-orf254 (7933aa, 67/76) | KS/AT/DH/KR/ACP-KS/ATm/DH/ER/KR/ACP-KS/AT/KR/ACP-KS/AT/KR/ACP-KS/AT/KR/ACP | CAJ88175 | Streptomyces ambofaciens ATCC 23877 | ||||||
| pks - 9 | – | s11-orf206 (7923aa, 54/66) | – | – | – | KS/AT/KR/ACP-KS/AT/KR/ACP-KS/AT/DH/KR/ACP-KS/AT/DH/KR/ACP-KS/AT/DH/KR/ACP | See Fig. 1g | BAC68129 | Streptomyces avermitilis MA-4680 = NBRC 14893 |
| s11-orf207 (1582aa, 54/65) | KS/AT/KR/ACP | WP_032769932 | Streptomyces sp. CNS654 | ||||||
| s11-orf208 (4703aa, 59/69) | KS/ATm/KR/ACP-KS/AT/KR/ACP-KS/AT/KR/ACP-TE | CAJ88175 | Streptomyces ambofaciens ATCC 23877 | ||||||
| pks - 10 | – | s26-orf59 (1260aa, 87/92) | – | – | – | KS/AT/ACP | ? | WP_030454772 | Herbidospora cretacea NRRL B-16917 |
| pks - 11 | – | – | – | – | s22-orf6 (4492aa, 74/80) | KS/AT/DH/KR/ACP-KS/ATm/KR/ACP-KS/AT/KR/ACP | See Fig. 1i | WP_042397188 | Streptacidiphilus carbonis NBRC 100919 |
| s22-orf5 (6067aa, 75/82) | KS/ATm/KR/ACP-KS/AT/KR/ACP-KS/ATm/KR/ACP-KS/AT/KR/ACP | WP_042397185 | Streptacidiphilus carbonis NBRC 100919 | ||||||
| s22-orf4 (3381aa, 56/65) | KS/ATm/DH/KR/ACP-KS/ATm/KR/ACP | WP_037075673 | Pseudonocardia spinosispora DSM 44797 | ||||||
| s22-orf3 (6184aa, 71/78) | KA/ATm/KR/ACP-KS/ATm/KR/ACP-KS/ATm/KR/ACP-KS/ATm/KR/ACP | WP_037075676 | Pseudonocardia spinosispora DSM 44797 | ||||||
| s22-orf2 (6426aa, 76/83) | KS/ATm/KR/ACP-KS/ATm/KR/ACP-KS/AT/KR/ACP-KS/ATm/DH/KR/ACP | WP_042408959 | Streptacidiphilus carbonis NBRC 100919 | ||||||
| s22-orf1 (> 4929aa, 77/83) | KS/ATm/KR/ACP-KS/ATm/DH/ER/KR/ACP-KS/ATm/DH… | WP_042408956 | Streptacidiphilus carbonis NBRC 100919 | ||||||
| pks - 12 | – | – | – | – | s02-orf765 (1258aa, 94/95) | KS/AT?/DH/ACP | ? | WP_030454772 | Herbidospora cretacea NRRL B-16917 |
Strain-specific gene clusters are in boldface. NRPS and PKS genes not completely sequenced are shown in italics. C, condensation; A, adenylation; T, thiolation; E, epimerization; MT, methyltransferase; CoL, CoA ligase; KS, ketosynthase; AT, acyltransferase incorporating malonyl-CoA, ATm, acyltransferase incorporating methylmalonyl-CoA; ATe, acyltransferase incorporating ethylmalonyl-CoA; AT?, acyltransferase whose substrate has not been determined by antiSMASH; DH, dehydratase; ER, enoylreductase; KR, ketoreductase; ACP, acyl carrier protein; TE, thioesterase; x, unpredicted amino-acid; mOrn, methyl ornithine; ?, lack of A domain. A domain amino-acid substrate predicted by antiSMASH is shown in subscripted letters