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. 2015 Jun 12;4(6):e001712. doi: 10.1161/JAHA.114.001712

Figure 2.

Figure 2

Zyxin is a crucial regulator of stretch-sensitive genes in VSMCs. A through C, Volcano plot analysis of changes in expression of genes in stretched WT and zyxin−/− VSMCs (fold expression vs calculated probability). Cells were stretched at 10% cyclic elongation for 6 hours and compared to unstretched (static) control cells. Genes that were differentially and significantly regulated (P<0.01) are displayed above the black solid line (n=3 for all experimental groups). 4718 genes were stretch-responsive in WT compared to only 315 in zyxin−/− VSMCs. D, Verification of 5 microarray target genes by real-time RT-PCR, *P<0.05 as indicated, n=3 for all experimental groups. ITGA8 indicates integrin α8; MMP13, matrix metalloproteinase 13; RGS5, regulator of G-protein signaling 5; RT-PCR, reverse transcriptase polymerase chain reaction; VSMCs, vascular smooth muscle cells; WT, wild type.