Figure 21.

Transient overexpression of LPP in zyxin^−/− VSMCs. A, Confocal immunofluorescence image of VSMCs from zyxin^−/− mice showing LPP localization at focal adhesions (in red). B, Zyxin^−/− VSMCs transfected with GFP as a transfection control. C, Exemplary image of zyxin-deficient VSMCs transfected with a plasmid expressing the coding sequence for LPP (Zyxin^−/− LPP). Scale bar represents 100 μm. D, Validation of LPP over-expression by Western blot analysis showing a 1.8-fold increase in LPP expression, n=4 for each group. GFP indicates green fluorescent protein; LPP, lipoma preferred partner; VSMCs, vascular smooth muscle cells.