Table 3.
Stretch-Regulated Zyxin-Maintained Pathways
| No. | Pathway (Source) Functional Description | Stretch vs Quiescent P (NES/FDR) | Stretch vs Quiescent/Zyxin Knockout P (NES/FDR) |
|---|---|---|---|
| 1 | Lymphocyte differentiation (GO pathway) | 0.553 (−0.95/0.824) | 0.002* (−1.72/0.364) |
| 2 | Positive regulation of T-cell activation (GO pathway) | 0.560 (−0.95/0.828) | 0.006* (−1.68/0.327) |
| 3 | T-cell differentiation (GO pathway) | 0.416 (−1.02/0.766) | 0.002* (−1.63/0.385) |
| 4 | Epithelial-cell proliferation (GO pathway) | 0.318 (−1.09/0.699) | 0.010* (−1.63/0.345) |
| 5 | Protein amino acid N-linked glycosylation (GO pathway) | 0.434 (−1.01/0.777) | 0.015* (−1.58/0.400) |
| 6 | Adaptive immune response (GO pathway) | 0.183 (−1.19/0.656) | 0.012* (−1.54/0.372) |
| 7 | Adaptive immune response superfamily domains (GO pathway) | 0.203 (−1.18/0.651) | 0.002* (−1.54/0.363) |
| 8 | Leukocyte differentiation (GO pathway) | 0.761 (−0.87/0.942) | 0.004* (−1.51/0.350) |
Selective GSEA-pathway analysis of stretch-sensitive genes in mouse aortic VSMCs (third column) and the role of zyxin therein deduced from changes in gene expression following zyxin-deficiency (fourth column). Zyxin-maintained pathways that respond in similar fashion in both groups are shown. Quiescent state indicates static VSMCs that were unstretched. All pathways are derived from the canonical pathway sets provided by KEGG (http://www.genome.jp/kegg/) and GO (pathways molecular function; http://www.geneontology.org). Only pathways found to be significantly altered (P≤0.02) by cyclic stretch and/or zyxin-deficiency have been included. NES are listed. Additionally, FDR have been included; a value of FDR ≤0.4 is regarded to be significant. These values are interpreted in the same manner as outlined in the legend to Table2. Raw data provided at NCBI GEO (http://www.ncbi.nlm.nih.gov/geo/query) using the sample number [GSE60447]. FDR indicates false discovery rates; GEO, Gene Expression Omnibus; GO, Gene Ontology; GSEA, Gene Set Enrichment Analysis; KEGG, Kyoto Encyclopedia of Genes and Genomes; NCBI, National Center for Biotechnology Information; NES, normalized enrichment scores; VSMCs, vascular smooth muscle cells.
P≤0.02.