Table 6.
Stretch-Regulated Zyxin-Repressed Pathways With Opposing Values of NES
| No. | Pathway (Source) Functional Description | Stretch vs Quiescent P (NES/FDR) | Stretch vs Quiescent/Zyxin Knockout P (NES/FDR) |
|---|---|---|---|
| 1 | M phase of mitotic cell cycle (GO pathway) | 0.000* (−1.92/0.024) | 0.000* (2.03/0.020) |
| 2 | Mitosis (GO pathway) | 0.000* (−1.94/0.024) | 0.000* (2.01/0.016) |
| 3 | M phase (GO pathway) | 0.000* (−1.96/0.024) | 0.000* (1.93/0.037) |
| 4 | Chromosome segregation (GO pathway) | 0.000* (−2.09/0.003) | 0.000* (1.92/0.035) |
| 5 | Mitotic cell cycle (GO pathway) | 0.008* (−1.48/0.285) | 0.000* (1.87/0.055) |
| 6 | Cell cycle phase (GO pathway) | 0.000* (−1.82/0.048) | 0.000* (1.83/0.079) |
| 7 | Response to oxidative stress (GO pathway) | 0.029 (−1.49/0.275) | 0.002* (1.77/0.137) |
| 8 | Cell division (GO pathway) | 0.000* (−1.75/0.080) | 0.000* (1.74/0.162) |
| 9 | Microtubule-based movement (GO pathway) | 0.816 (−0.81/0.967) | 0.002* (1.67/0.297) |
| 10 | Sister chromatid segregation (GO pathway) | 0.045 (−1.59/0.195) | 0.006* (1.66/0.300) |
| 11 | Mitotic sister chromatid segregation (GO pathway) | 0.024 (−1.57/0.209) | 0.009* (1.65/0.277) |
| 12 | Cytoskeleton-dependent intracellular transport (GO pathway) | 0.862 (−0.79/0.975) | 0.002* (1.65/0.265) |
| 13 | Cell cycle process (GO pathway) | 0.000* (−1.46/0.318) | 0.000* (1.54/0.476) |
| 14 | Starch and sucrose metabolism (KEGG) | 0.063 (−1.43/0.333) | 0.008* (1.78/0.147) |
Selective GSEA-pathway analysis of stretch-sensitive genes in mouse aortic VSMCs (third column) and the role of zyxin therein deduced from changes in gene expression following zyxin-deficiency (fourth column). Zyxin-repressed pathways that respond in opposite fashion in the 2 groups are shown. Quiescent state indicates static VSMCs which were unstretched. All pathways are derived from the canonical pathway sets provided by KEGG (http://www.genome.jp/kegg/) and GO (pathways molecular function; http://www.geneontology.org). Only pathways found to be significantly altered (P≤0.02) by cyclic stretch and/or zyxin-deficiency have been included. NES are listed. Additionally, FDR have been included; a value of FDR ≤0.4 is regarded to be significant. These values are interpreted in the same manner as outlined in the legend to Table2. Raw data provided at NCBI GEO (http://www.ncbi.nlm.nih.gov/geo/query) using the sample number [GSE60447]. FDR indicates false discovery rates; GEO, Gene Expression Omnibus; GO, Gene Ontology; GSEA, Gene Set Enrichment Analysis; KEGG, Kyoto Encyclopedia of Genes and Genomes; NCBI, National Center for Biotechnology Information; NES, normalized enrichment scores; VSMCs, vascular smooth muscle cells.
P≤0.02.