Table 8.
In Silico Promoter Analysis of the 3 MRTF-A-Regulated Zyxin-Dependent Candidate Genes for Putative SRF Binding Sites
| Gene | Matrix | Start | End | C.S | M.S | Sequence |
|---|---|---|---|---|---|---|
| CyclinE2 | V$SRF.04 | −2464 | −2482 | 1 | 0.865 | ctaacccgtTATGgcaaga |
| ITGA8 | V$SRF.03 | −2524 | −2542 | 1 | 0.805 | cttcacctatATGGactgc |
| V$SRF.05 | −1053 | −1071 | 1 | 0.816 | ctagcctgagAAGGctgtg | |
| V$SRF.02 | −381 | −399 | 1 | 0.868 | tgttcCATAtgcggcgaga | |
| V$SRF.04 | −380 | −398 | 1 | 0.925 | ctcgccgcaTATGgaacac | |
| MMP13 | V$SRF.04 | −2915 | −2933 | 0.901 | 0.87 | tggaccacaTAAGacccaa |
| V$SRF.02 | −1737 | −1755 | 1 | 0.851 | gggctCATAtcagggccca | |
| V$SRF.04 | −1736 | −1754 | 1 | 0.872 | gggccctgaTATGagccct | |
| V$SRF.05 | −1423 | −1441 | 1 | 0.838 | ttgacctcaaAAGGgggag |
In silico promoter analysis of the 3 MRTF-A-regulated zyxin-dependent candidate genes for putative SRF binding sites. Putative SRF binding sites are tabulated. A maximum C.S is obtained when there is a strong match between highly conserved bases of the matrix and the input sequence. A good match to the matrix or the M.S has a value >0.80. Promoter sequences ≈4000 bp upstream of the transcription start site were analyzed for this purpose. C.S indicates core similarity; ITGA8, integrin α8; M.S, matrix similarity; MMP13, matrix metalloproteinase 13; MRTF-A, myocardin-related transcription factor A; SRF, serum response factor.