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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Thromb Haemost. 2015 Apr 23;13(6):1064–1072. doi: 10.1111/jth.12901

Fig. 3.

Fig. 3

Bilirubin oxidase reverses the inhibitory activity of bilirubin on the cleavage of multimeric VWF by recombinant ADAMTS13. A. Pretreatment of the reaction mixture containing plamsa VWF, rA13, and unconjugated bilirubin in the absence (−) or presence (+) of increasing concentrations of bilirubin oxidase (0-8 U/ml) reversed the inhibitory activity on the cleavage of multimeric VWF in a concentration-dependent manner, determined by Western blot after separation with either 1% agarose gel electrophoresis (top) or 5% SDS-polyacrylamide gel electrophoresis (bottom). B. The quantitative data of the cleavage product (~350 kDa) shown in the bottom of panel A represent the means ± standard errors from three independent experiments. The arrow indicates 100% of ADAMTS13 activity in the absence of bilirubin and bilirubin oxidase.