. Author manuscript; available in PMC: 2016 Jun 1.

Published in final edited form as: J Thromb Haemost. 2015 Apr 23;13(6):1064–1072. doi: 10.1111/jth.12901

Table 1.

Kinetics of binding of unconjugated bilirubin to GST-rVWF73-H, rADAMTS13, and VWF

	k_a (1/Ms)	k_d (1/s) ×10⁻³	R_max (RU)	K_D (μM)
GST-rVWF73-H	159.8 ± 86.0	4.2 ± 2.1	216.0 ± 200.0	57.5 ± 80.7
rADAMTS13	208.8 ± 25.2	3.6 ± 0.61	189.3 ± 69.3	17.5 ± 4.0
VWF	169.9 ± 84.9	2.4 ± 1.0	301.0 ± 264.7	29.6 ± 40.1

The kinetic parameters were determined using 1:1 Langmuir fit model. k_a and k_d respresent the rates of association and dissociation; K_D is the ratio of kd/ka, the association rate constant. The data are the means ± standard deviation (SD) of 4 repeated experiments