Table II.
In vitro assay | Available growth area (cm2) | Seeding density | Duration | Endpoint |
---|---|---|---|---|
CFE | 28.2 | 300 | 7 days | % of colonies |
Morphological transformation | 28.2 | 30,000 | 21 days | Foci count |
Cell proliferation | 0.33 | 5000 | 24 h, 48 h | WST-1 absorbance |
Trypan exclusion assay (proliferation) | 28.2 | 30,000 | 24 h, 48 h | Live cell count |
Soft agar colony formation | 9.6 | 10,000 | 14 days | Colony count |
Invasion matrigel chemotaxis transwell | 0.3 | 15,000 | 48 h | Invaded cell count |
Migration chemotaxis transwell | 0.3 | 30,000 | 24 h | Migrated cell count |
HUVEC angiogenesis of SAEC conditioned medium | 2.0 | 45,000 | 16 h | Tube node count |
Whole-genome expression profile | 28.2 | 1,000,000 | 24 h | mRNA expression |