Table II.

In vitro assay conditions and endpoints used to assess neoplastic-like behaviour in human SAECs subchronically exposed to ultrafine carbon black, SWCNTs, MWCNTs and crocidolite asbestos.

In vitro assay	Available growth area (cm2)	Seeding density	Duration	Endpoint
CFE	28.2	300	7 days	% of colonies
Morphological transformation	28.2	30,000	21 days	Foci count
Cell proliferation	0.33	5000	24 h, 48 h	WST-1 absorbance
Trypan exclusion assay (proliferation)	28.2	30,000	24 h, 48 h	Live cell count
Soft agar colony formation	9.6	10,000	14 days	Colony count
Invasion matrigel chemotaxis transwell	0.3	15,000	48 h	Invaded cell count
Migration chemotaxis transwell	0.3	30,000	24 h	Migrated cell count
HUVEC angiogenesis of SAEC conditioned medium	2.0	45,000	16 h	Tube node count
Whole-genome expression profile	28.2	1,000,000	24 h	mRNA expression