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. 2015 Oct 8;13:259. doi: 10.1186/s12916-015-0489-y

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© Repetto et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — s-RNY1-5p is stable in extracellular environment. a Northern blot analysis showing the stability of s-RNY1-5p and U6 snRNA in the medium of bone marrow-derived macrophages stimulated with 1 μM of staurosporine at the indicated time points. Total RNA was isolated from the medium. b, c RT-qPCR analysis from three coronary artery disease patients showing the stability of s-RNY1-5p in the serum in either prolonged room temperature incubation (b) or different cycles of freeze-thaw as indicated (c). Data were normalized by using the synthetic non-mammalian cel-miR-39, which was added to the samples before the RNA extraction. Each ΔCt (corresponding to Ct^s-RNY1-5p-Ct^cel-miR-39) is the average of three technical replicates