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. 2015 Oct 9;13:66. doi: 10.1186/s12951-015-0127-z

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© Sánchez-Sánchez et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Cytochrome P450 activity assay in human cervix carcinoma cell line (HeLa). Staining with DAPI show nuclei of HeLa cells labeled as “n”, panels a and d. Endogenous CYP activity over BFC reagent was visualized in HeLa cells as observed in panel b. CYP activity of transfected VLPs-CYP nanoparticles in HeLa cells is shown in panel e. Overlay of DAPI and BFC localize the CYP activity in the cytoplasm of HeLa cells (white arrows), panels c and f. Scale bar represents 20 μm. Cells were visualized with a ×63 (DIC), 1.4 N.A. planapochromatic oil immersion objective