Table 1.

Comparison between expression systems for the encapsulation of CYPBM3 inside P22

Expression system (plasmids)	CYP/capsida	Total CYPb Abs280 (µM)	Active CYP CO assayc (µM)	% active CYPd (CO/Abs280)
Co-expression (pETDuet)	156.0 (±0.4)	123.5	9.2	7
Differential expression 1 (pBAD + pRSF)	129.5 (±0.1)	135.3	31.8	23
Differential expression 2 (pBAD + pRSF)	109.7 (±2.8)	123.7	42.9	35

^aDetermined by Eq. 1

^bTotal CYP determined using an extinction coefficient at 280 nm assuming a molar extinction coefficient ε₂₈₀ = 44,920 M⁻¹ cm⁻¹ for coat protein and ε₂₈₀ = 52,830 M⁻¹ cm⁻¹ for CYP-SP (theoretically calculated using ProtParam, Gasteiger, 2005). The concentration of protein was calculated using the Lambert–Beer equation, Abs_T = C_CP·ɛ_CPl + C_CYP-SP·ɛ_CYP-SPl as described previously [15]

^cActive CYP determined by the formation of CO-CYP complex in reducing medium with a extinction coefficient of ε₄₅₀ = 0.091 nM⁻¹cm⁻¹ [26]

^dActive/total CYP ratio expressed in percentage