Figure 3.

Anti-Aß scFvs are secreted and co-localize with Aß42 in S2 cells. (A and B) ScFvs and Aß42 are secreted in cultured S2 cells. In S2 cells transfected with scFv or Aß42 constructs, both scFv9 and scFv42.2 (A) and Aß42 (B) are detected in the culture medium by western blot. Equivalent amounts of cell lysates were used to detect Tubulin as loading control from each culture. (C–H) Subcellular distribution of Aß42 and anti-Aß scFvs in cultured S2 cells. Co-transfection of Aß42 and scFv9 (C–E) or scFv42.2 (F and G) shows perinuclear co-localization corresponding to ER and vesicles of the secretory pathway (arrows).