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. 2015 Aug 7;24(21):6093–6105. doi: 10.1093/hmg/ddv321

Figure 7.

Figure 7.

Anti-Aß scFvs are neuroprotective without affecting Aβ42 aggregation. (A–E) Accumulation of apoptotic cells in the Kenyon cell clusters at Day 20 in flies expressing the indicated constructs under the control of OK107-Gal4. Control flies expressing LacZ accumulate no TUNEL-positive cells (arrows) (A). Flies expressing Aß42 and LacZ accumulate ∼20 TUNEL-positive cells per Kenyon cell cluster (B). Flies co-expressing scFv9, scFv42.2 or both show significantly fewer apoptotic cells per Kenyon cell cluster (C–E). (I) Graphic representation of the average of apoptotic cells per cluster. Values are expressed as average ± standard error. (F–H and J–N) Accumulation of thioflavin-S in the Drosophila brain. Control flies expressing LacZ in the Kenyon cells (arrows) show no fluorescent signal (F and J). Flies co-expressing Aß42 and LacZ show strong and specific fluorescence (G and K). Co-expression of Aß42 and scFv9, scFv42.2 or both shows no effect on the thioflavin-S signal (L-N). (H) Quantification of the fluorescent signal from thioflavin-S shows significant increase in intensity in flies co-expressing Aß42 and scFv42.2. **P < 0.01; ****P < 0.0001.