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. 2015 Aug 7;24(21):6093–6105. doi: 10.1093/hmg/ddv321

Figure 8.

Figure 8.

Anti-Aß scFvs suppresses Aβ42 neurotoxicity without reducing Aß42 levels. (A) Western blot in 1-day-old flies and quantification. Flies co-expressing Aß42 and the anti-Aß scFvs show similar levels of Aß42 as those co-expressing LacZ. Only flies co-expressing scFv42.2 showed slightly higher, but not significant levels of Aß42. A representative blot is shown, but the Aß42 quantification was done from three independent replicates. (B and C) Quantification of total Aβ42 and the SDS fraction at Days 1 (D1) and 20 (D20) by ELISA. (B) In young flies, co-expression of scFv9 and scFv9+42.2 led to a slight but significant reduction of Aß42 (n = 5). In 20-day-old flies, all the anti-Aß scFv combinations increase the levels of Aß42, but only scFv42.2 and scFv9+42.2 are significant. (C) The levels of Aß42 in the SDS fraction are mostly unchanged by the expression of anti-Aß scFvs, except for a modest increase in scFv9 at Day 1.