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. 2015 Aug 6;24(21):6106–6117. doi: 10.1093/hmg/ddv322

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© The Author 2015. Published by Oxford University Press

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — No dominant toxicity of human or Drosophila Vps35 variants. (A) ClustalW2 Alignment of Vps35 amino acid sequence from human (hVps35) and Drosophila (dVps35) with the missense mutations highlighted. (B) Immunoblot of human Vps35 transgenes raised at different temperatures to monitor relative expression levels. (C) Transgene expression does not affect lifespan when expressed ubiquitously with da-GAL4 [all not significant (ns) compared with control, Log-rank test, n = 40–100 animals]. (D) No overt phenotype is seen when the transgenes were expressed in the compound eye by GMR-GAL4. (E–G) Ubiquitous expression does not affect climbing ability with age [all not significant (ns) compared with control]. n = 25–40 (E), 20–40 (F) and 10–40 (G). Histograms show mean ± SEM. Statistical analysis was one-way analysis of variance (ANOVA) with Bonferroni's correction. Control genotypes are heterozygous GAL4 driver line alone.