Table 1.
A combination of three non-overlapping HTT exon1 peptides is highly immunogenic in HD mutant and control mice
| Immunogen | Mouse ID | zQ175 |
R6/1 | WT control |
|||
|---|---|---|---|---|---|---|---|
| ELISA | WB | ELISA | WB | ELISA | WB | ||
| AA1-17 peptide | 1 | >2000 | 2000 | 600 | 2000 | ND | ND |
| 2 | >2000 | 5000 | 600 | 2000 | ND | ND | |
| 3 | >2000 | 2000 | 200 | 2000 | ND | ND | |
| 4 | >2000 | 5000 | 225 | 2000 | ND | ND | |
| 5 | 900 | 2000 | 225 | 2000 | ND | ND | |
| AA49-60 peptide | 1 | >2000 | <500 | 375 | <200 | ND | ND |
| 2 | ND | ND | 75 | <200 | ND | ND | |
| 3 | 1000 | <500 | <50 | <200 | ND | ND | |
| 4 | 100 | <200 | 75 | <200 | ND | ND | |
| 5 | <50 | <200 | <50 | <200 | ND | ND | |
| AA74-88 peptide | 1 | >2000 | 2000 | 900 | <200 | ND | ND |
| 2 | 1200 | <200 | ND | <200 | ND | ND | |
| 3 | 350 | 200 | 150 | <200 | ND | ND | |
| 4 | <50 | 200 | <50 | <200 | ND | ND | |
| 5 | <50 | <200 | 75 | <200 | ND | ND | |
| Combined triple Peptidesa | 1 | >5000 | 2000 | 1200 | 2000 | 750 | 2000 |
| 2 | 5000 | 2000 | 2000 | 2000 | 5000 | 2000 | |
| 3 | 5000 | 2000 | 1000 | 2000 | 2000 | 2000 | |
| 4 | >1000 | 2000 | 250 | 2000 | 5000 | 2000 | |
| 5 | 1000 | 2000 | ND | ND | 500 | 2000 | |
| HTTexon1-Q46 protein | 1 | 270 | ND | 250 | ND | 250 | ND |
| 2 | 250 | ND | 350 | ND | 500 | ND | |
| 3 | 500 | ND | 90 | ND | 500 | ND | |
| 4 | 500 | ND | 220 | ND | ND | ND | |
| 5 | 250 | ND | 500 | ND | ND | ND | |
Anti-Htt antibodies in sera of immunized mice were measured by ELISA or Western blotting. Antibody titers >1000 are used to indicate relative responses, and are shaded in gray. Serum antibody titer is defined as the reciprocal of the dilution whose absorbance is 3× that of negative controls.
ND, Not determined.
aCombination of the three peptides above.