Table 2.

The HTT N586-Q82 plasmid is more immunogenic than the HTT exon1-Q97 plasmid in HD mutant and control mice

Immunogen	Mouse ID	zQ175		R6/1		WT control
		ELISA	WB	ELISA	WB	ELISA	WB
HTTexon1-Q97 plasmid	1	40	<200	50	<200	<25	<200
	2	35	<200	<25	<200	<25	<200
	3	25	<200	40	<200	70	<200
	4	40	<200	45	<200	30	<200
	5	45	<200	<25	<200	25	<200
HTTexon1-Q97 + IL-4 plasmids	1	100	<200	<25	<200	25	<200
	2	100	<200	<25	<200	300	<200
	3	40	<200	<25	<200	90	<200
	4	<25	<200	25	<200	45	<200
	5	175	ND	25	<200	125	<200
HTT N586-Q82 plasmid	1	900	2000	100	2000	4000	5000
	2	600	<200	900	2000	1600	5000
	3	250	<200	100	2000	4000	5000
	4	ND	ND	<25	2000	800	5000
	5	350	2000	<25	<200	900	5000
	6					2000	5000
HTT N586-Q82 + IL-4 plasmids	1	25	<200	150	200	350	200
	2	80	<200	<25	<200	>500	5000
	3	400	<200	1900	200	>500	5000
	4	60	<200	100	<200	>500	5000
	5	25	<200	150	<200	>500	5000
	6					<25	<200
	7					>500	5000
HTTexon1-Q97 plasmid prime + triple peptide boost	1	35	ND	90	ND	100	ND
	2	240	ND	35	ND	<25	ND
	3	<25	ND	<25	ND	450	ND
	4	45	ND	<25	ND	50	ND
	5	<25	ND	<25	ND	175	ND
HTT N586-Q82 plasmid prime + triple peptide boost	1	500	ND	35	ND	65	ND
	2	200	ND	<25	ND	50	ND
	3	80	ND	<25	ND	50	ND
	4	45	ND	<25	ND	60	ND
	5	ND	ND	<25	ND	375	ND

Anti-Htt antibodies in sera of immunized mice were measured by ELISA or Western blotting, as in Table 1. Antibody titers >500 are shaded in gray, since overall readings were lower.

ND, Not determined.