Figure 5.
Measurement of stereociliary lengths in Dfnb31neo/neo and Dfnb31wi/wi cochlear hair cells. (A and B) Dfnb31wi/wi (wi) but not Dfnb31neo/neo (neo) mice at P4 had short stereocilia in IHCs (A) and OHCs (B). (C) Both Dfnb31wi/wi and Dfnb31neo/neo mice at P45 had short stereocilia in IHCs, although Dfnb31neo/neo IHC stereocilia were longer than Dfnb31wi/wi IHC stereocilia. (D) Dfnb31wi/wi but not Dfnb31neo/neo mice at P45 had short stereocilia in OHCs. Representative SEM images of stereocilia from each genotype at P4 and P45 are shown on the right of bar charts. Stereocilia in the longest row next to the kinocilium at P4 or in the middle of the row at P45 were measured. Numbers of cells and mice analyzed are listed in the bottom of bars before and after slashes, respectively. Student's t-tests (two-tail) were performed. Error bars, standard error of the mean. **P < 0.01.