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. 2015 Oct 5;10:6243–6256. doi: 10.2147/IJN.S90733

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© 2015 Zhang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Cytotoxic effects of silver nanoparticles in Leydig cells and Sertoli cells.

Notes: Leydig cells and Sertoli cells were treated with various concentrations of silver nanoparticles for 24 hours, and cytotoxicity was determined using 3(4-5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. The results represent the means of three independent experiments, and the error bars show the standard errors of the means. The cytotoxicity of silver nanoparticles-treated groups was significantly different from that of the control group, as determined by the Student’s t-test (P<0.05). (A) The MTT assays of Leydig cells. (B) The MTT assays of Sertoli cells. (C) LDH assays of Leydig cells. (D) LDH assays of Sertoli cells. *P<0.05, **P<0.01.

Abbreviation: au, arbitrary unit.