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. 2015 Oct 5;10:6243–6256. doi: 10.2147/IJN.S90733

Figure 7.

Figure 7

Pachytene analysis of germ cells from silver nanoparticles-exposed males and controls.

Notes: (A) Representative immunostaining image of MLH1 in pachytene spermatocytes. Double immunostaining with antibodies against SYCP3 (red) and MLH1 (green) for detection of the synaptonemal complex and sites of recombination. (B), (D), and (F) Distribution of MLH1 foci. The proportion of Sertoli cells with zero, one, two, or three MLH1 foci (exchange) in pachytene cells from silver nanoparticles-exposed males at postnatal day PND28, PND42, and PND60. (C), (E), and (G). MLH1 foci per cell from silver nanoparticles-exposed males at PND28, PND42, and PND60. **P<0.01.

Abbreviation: SCs, Sertoli cells.