Table 2.

Preparation conditions for rhEPO-loaded PEG–PLA micelles

Batch	PEG–PLAa	PEG–PLA concentration (mg/ml)	Rotation speed (rpm)	pH of PBS
1	PEG5000–PLA3900	1	1300	7.4
2	PEG5000–PLA5800	1	1800	7.4
3	PEG5000–PLA7600	1	1800	7.4
4	PEG5000–PLA5800	1	1000	7.4
5	PEG5000–PLA5800	1	2300	7.4
6	PEG5000–PLA3900	2	1800	7.4
7	PEG5000–PLA3900	3	1800	7.4
8	PEG5000–PLA3900	5	1800	7.4
9	PEG5000–PLA3900	2	1800	2.5
10	PEG5000–PLA3900	2	1800	3.6
11	PEG5000–PLA3900	2	1800	3.8
12	PEG5000–PLA3900	2	1800	4.0
13	PEG5000–PLA3900	2	1800	4.5
14	PEG5000–PLA3900	2	1800	7.4
15	PEG2000–PLA3800	2	1800	3.8
16	PEG10000–PLA3600	2	1800	3.8

Note:

^aPolymer terminology, whereas the numbers after PEG and PLA and M_n (determined by ¹H NMR spectroscopy) of PEG and PLA respectively.

Abbreviations: rhEPO, recombinant human erythropoietin; PEG–PLA, poly(ethylene glycol)–poly(d,l-lactide); PBS, phosphate buffered saline.