Table 3.
Characteristics of rhEPO-loaded PEG–PLA micelles
| Batch | Particle size (nm) | PDI | Zeta potential (mV) | EE (%) | Aggregation (%) |
|---|---|---|---|---|---|
| 1 | 73.4 ± 3.6 | 0.23 | −3.78 ± 2.12 | 8.2 ± 2.1 | 5.16 ± 1.03 |
| 2 | 137.5 ± 5.3 | 0.22 | 4.65 ± 1.65 | 7.4 ± 3.4 | 3.35 ± 1.52 |
| 3 | 177.8 ± 6.2 | 0.31 | 4.55 ± 1.38 | 3.1 ± 1.2 | 23.02 ± 4.37 |
| 4 | 196.4 ± 4.9 | 0.22 | 1.14 ± 1.65 | 10.4 ± 3.1 | 4.70 ± 1.46 |
| 5 | 187.6 ± 3.2 | 0.25 | −3.06 ± 2.39 | 3.3 ± 1.2 | 3.68 ± 1.21 |
| 6 | 80.4 ± 1.6 | 0.22 | 0.68 ± 1.26 | 14.7 ± 32 | Trace* |
| 7 | 83.4 ± 3.1 | 0.27 | 0.92 ± 1.53 | 5.2 ± 1.6 | 3.38 ± 0.95 |
| 8 | 77.4 ± 1.6 | 0.43 | 0.43 ± 4.29 | N | N |
| 9 | 67.2 ± 2.6 | 0.19 | −0.46 ± 1.14 | 47.2 ± 3 5 | Trace* |
| 10 | 70.1 ± 1.7 | 0.21 | −0.75 ± 0.96 | 57.5 ± 4.3 | Trace* |
| 11 | 71.5 ± 2.3 | 0.15 | 0.21 ± 1.32 | 71.5 ± 1.6 | Trace* |
| 12 | 72 3 ± 1.5 | 0.19 | −0.54 ± 0.95 | 58.3 ± 1.8 | Trace* |
| 13 | 69.8 ± 1.8 | 0.25 | 1.86 ± 0.91 | 60.4 ± 4.6 | Trace* |
| 14 | 80.2 ± 2.1 | 0.23 | −1.35 ± 1.46 | 20.6 ± 3.4 | Trace* |
| 15 | 73.4 ± 2.8 | 0.24 | 1.28 ± 0.98 | 54.9 ± 3.2 | Trace* |
| 16 | 131.2 ± 4.3 | 0.28 | −0.76 ± 1.23 | 79.2 ± 4.9 | Trace* |
Notes:
*
Trace meant below the limit of detection. N demonstrated didn’t detect.
Abbreviations: rhEPO, recombinant human erythropoietin; PEG–PLA, poly(ethylene glycol)–poly(d,l-lactide); PDI, polydispersity index; EE, encapsulation efficiency.